Brent Supplement Mixture (BSM) is recommended for use with the addition in DOB or DOBA and carbon source for the growth of Saccharomyces cerevisiae. It is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae. It is to be mixed with yeast nitrogen base (YNB), Ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of budding yeast.