This media is formulated as per American Public Health Association for presumptive identification and confirmation of coliform bacteria. During examination of water samples, growth from presumptive positive tubes showing gas in Lactose Broth (M026) or Lauryl Tryptose Broth (M080) is inoculated in Brilliant Green Bile Broth 2% wherein gas formation within 48 ± 2 hours confirms the presumptive test