Esculin Mannitol Agar is a selective and differential media whaich differentiation of Enterococcus and Staphylococcus based on mannitol fermentation and esculin hydrolysis. Esculin hydrolysis is shown by Enterococcus species. Enterococcus species hydrolyse esculin and hence black precipitate is observed around the colonies. S.aureus ferment mannitol and produce yellow coloured colonies surrounded by yellow zones. Coagulase-negative strains of S.aureus are usually mannitol non-fermenters and therefore produce pink to red colonies