Fluconazole Testing Medium (Twin Pack) is recommended for fluconazole susceptibility testing using Candida species. To carry out standardization of antifungal drug susceptibility assays as a standard reference method, CLSI had recommended broth macro dilution testing of yeasts. Fluconazole Testing Medium is a chemically defined medium specifically developed for the in-vitro testing of fluconazole by using Candida species. Inhibitory concentration values obtained by using this medium correlate well with the clinical outcome.