HiCromeâ„¢ EC Broth w/RUG 500G

Escherichia coli can be reliably detected with this media that contain a chromogenic or fluorogenic substrate for beta-glucuronidase, an enzyme that occurs almost exclusively in E. coli . Resorufin-beta-D-glucuronic acid methyl ester (RUG) is a highly sensitive chromogenic and fluorogenic indicator for Escherichia coli RUG is more specific and does not require fluorescent detection. The released dye Resorufin itself gives intense pink color which can be visually detected. Additional confirmation can be done by observation of fluorescence under uv light .