Minimal Broth, Davis 500G

Minimal Broth, Davis is recommended for the isolation and characterization of nutritional mutants of Escherichia coli. The nutritional supplements to be added to minimal medium depends upon the type of mutant to be screened as for amino acids, vitamins, nucleic acids or other substances. This can be achieved by addition of vitamin assay casamino acids plus tryptophan or a mixture of water soluble vitamins, yeast or nucleic acid extracts.