MRS Selective Agar Base w/Clindamycin -Ciprofloxacin is recommended for the selective cultivation of Lactic acid bacteria from food. The composition of this medium are as per the specifications laid down in ISO 20128:2006. Lactobacilli are microaerophilic and generally require layer plates for aerobic cultivation on solid media. When the medium is set, another layer of un-inoculated MRS Agar is poured over the surface to produce a layer plate. Lactobacilli isolated on MRS Agar should be further confirmed biochemically. Clindamycin and ciprofloxacin helps in the elimination of contaminating flora.