PKU Test Agar w/ Thienylalanine is recommended for estimation of phenylalanine in blood for detection of Phenylketonuria (PKU).This medium was developed on the observation that Bacillus subtilis is normally inhibited in presence of β-2-thienylalanine but grows well when L-phenylalanine is added to the medium. Phenylalanine neutralizes the β-2-thienylalanine and allows bacteria to grow. The phenylalanine level can be read to determine the level of amino acid in blood.