Starch M-Protein Agar is recommended for isolation and propagation of Actinomycetes from soil and water. This medium is recommended by APHA for the growth of Actinomycetes species. M-protein serves as nitrogen source. Starch serves as a carbon source used as a substrate in anaerobic fermentation. Sodium chloride maintains osmotic balance. Dipotassium phosphate provides the buffering system. The sulphate serve as source of sulphur and metallic ions. Glycerol serves as an additional source of carbon.