B12 Assay Agar (Using Escherichia coli Mutant Culture) Harrison et.al Medium 100G

Recommended for microbiological assay of Vitamin B12 by plate method using Escherichia coli mutant 113-3 Davis ATCC 11105 as a test organism. The medium contains all essential nutrients except Vitamin B12. The addition of B12 in specified increasing concentration gives a growth response which can be measured with a zone reader.