Bile Esculin Azide Agar, Granulated is recommended for isolation and presumptive identification of faecal Streptococci. The composition and performance criteria of this medium are as per the specifications laid down in ISO 7899-2:2000. Bile and sodium azide inhibits other accompanying bacteria. Esculetin gives a dark brown or black complex which forms a zone of black precipitate around the colonies due to esculin hydrolysis.