Bromo Cresol Purple Indicator is recommended as a protein probe in spectrophotometric determination of serum albumin. Bromocresol purple or 5′,5″-dibromo-o-cresolsulfophthalein, is a dye of the triphenylmethane family. It is a pH indicator. It is colored yellow below pH 5.2 and violet above pH 6.8 . Bromocresol Purple is a water soluble dye used as a protein probe. It has been used in the spectrophotometric determination of serum-albumin . In microbiology, it is used for staining dead cells based on their acidity and for the isolation and assaying of lactic acid bacteria