Differential Agar for Group D Streptococci is used for the differentiation and identification of Group D Streptococci. Most strains of Group D Streptococci are now classified in the genus Enterococcus. These organisms grow on media with high salt content and are usually non-haemolytic, but sometimes show alpha or beta-haemolysis. It can withstand heat at 60°C for 30 minutes, a distinguishing feature from other streptococci, and also grow within a wider temperature range (10-45°C). They ferment sugars with acid production.