Esculin Iron Agar 500G

Esculin Iron Agar is recommended for verifying enterococcal colonies on membrane filters through which water samples have been filtered and which have been incubated on M-Enterococcus Agar, Modified (M1048).used in conjunction with M-Enterococcus Agar, Modified, (M1048) for verification of enterococcal colonies in fresh and marine recreational water, as recommended by APHA. Esculin in the medium is hydrolyzed by Enterococci to form esculetin and dextrose. Esculetin reacts with the iron salt (ferric ammonium citrate) and produces a dark brown to black complex, which appears around the colonies.