Gelatin Phosphate Salt Agar (GPS Agar) is recommended for cultivation and characterization of Vibrio cholerae from food. It is a non-selective medium formulated as per APHA and used for plating enrichment cultures of V.cholerae obtained from seafoods or vegetables. Gelatinase enzyme producing Vibrio's degrade gelatin and form small colonies, which are transparent with a cloudy halo. Gelatinase negative organisms show a satellite growth and may surround the colonies of V.cholerae on this medium.