Pantothenate Assay Medium, AOAC 100G

Pantothenate Assay Medium, AOAC is used for microbiological assay of pantothenic acid or its salts using Lactobacillus plantarum ATCC 8014 as the test organism. A dehydrated medium free from pantothenic acid or pantothenate but containing all other nutrients and vitamins essential for the cultivation of L. plantarum ATCC 8014 as recommended by AOAC. To obtain a standard curve pantothenate is added in particular increasing concentrations giving a growth response that can be turbidimetrically measured. The concentration of pantothenate is calculated by interpretation on the standard curve.