PPLO Modified Broth Base w/o CV is used for isolation and cultivation of Mycoplasma species (pleuropneumonia like organisms). Mostly the Mycoplasma species are aerobic or facultatively anaerobic but some are microaerophilic. Few are anaerobic saprophytic Mycoplasma which grow best at 22-35°C while pathogenic strains grow at 35°C. Mycoplasma gallinarium, Mycoplasma bovis, Mycoplasma pneumoniae gives good to luxuriant growth onto this medium.