Proline 1VL

Proline discs are used for amino acid decarboxylation test. To determine amino acid decarboxylation, the Proline disc (DD052) is added in the Decarboxyalse Broth Base, Moeller (M393) which is used as a negative control for studying decarboxylation or as a base for the addition of amino acids. The test organism is inoculated into the broth containing the Proline disc (DD052). The inoculated tubes are overlaid with sterile mineral oil and incubated at 35-37°C for up to 4 days. Positive Test: Colour of the medium changes from yellow to purple. Negative Test: Colour of the medium changes to yellow or there is no change