RPF Agar Base is recommended for the enumeration of coagulase positive Staphylococci from food and animal feeding stuffs. The composition and performance criteria are in accordance with ISO 6888-2:1999 & / Amd 1:2003. This medium works on same principle as Baird Parker Agar with addition of Fibrinogen Plasma together with Trypsin and Potassium Tellurite as single supplement. The medium is used for isolation and enumeration of Staphylococci in food and other material since it allows a good differentiation of coagulase positive strains.Staphylococci appera as white to grey-black coloured colony surrounded by an opaque zone.