Tryptose Agar is recommended for isolation, cultivation and differentiation primarily of Brucella, but also of Streptococci, Pneumococci, Meningococci and other pathogenic microorganisms.This medium is recommended by APHA and FDA as a general purpose media for cultivation of wide variety of organisms. It can also be supplemented with defibrinated blood (sheep, horse) to prepare blood agar for the isolation of fastidious organisms like Brucella.